FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4922527
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC23650.026.3762781
CTATACT22450.025.8787044
TATGGCG10200.024.36350816
TCGTCAA7100.024.15323360
GATTTCG7750.023.93404841
GTACCGT2250.023.3319326
TATACTG25100.023.2854525
TTCGTCA7700.023.18066244
ATTTCGT7850.022.73725542
GTAGGAC22450.022.4521883
TTTCGTC8050.022.17235643
CCTATAC18450.021.438513
TACACTG39850.021.4290775
CTACACT32050.020.9664064
CGTCAAG7700.020.90762161
TCGTCAT7950.020.69145
CTAGGAC23300.020.5815093
TAGGACC22500.020.2214164
CGTCATT9100.019.99946
TCTATAC21800.019.9102953
Produced by FastQC (version 0.11.5)