FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19789661
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG87150.023.4514735
CTATACT78200.023.1381864
CTACACT125000.021.8948634
TACACTG157500.021.575625
GTCCTAC84150.020.9886861
GTAGGAC87400.020.3451233
TATGGCG37250.020.01415416
CCTATAC66800.019.3880143
CCTACAC120550.019.0187573
CTACCCT130000.018.4413494
TAGACTG98100.018.0512035
TACACCG20850.017.7919865
TGTACTG130650.017.4915545
GTCCTAG99300.017.4335781
GATTTCG26800.017.36863141
GTGTAGC107350.017.3014561
GTCTAGG88150.017.2932151
TACACAG216450.017.2516845
GTATAAG65050.017.2389771
TCGTCAA25250.017.18670560
Produced by FastQC (version 0.11.5)