FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30643006
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT106600.022.0947324
CGAAGTA299950.021.36385570
TATACTG110000.020.7116625
CTACACT176900.020.3967884
TACACTG211400.020.1139775
TAGACTG142950.018.165465
CCTACAC186550.018.1057623
CCTATAC99550.018.0720183
GTCCTAC108850.017.9001481
GTAGGAC116000.017.5006733
TAGGACT141600.017.473794
TACACCG33550.017.2114735
GTGTAGC159850.016.8367731
GTCCTAG155000.016.7531621
ACGAAGT403850.016.6647770
CTAGACT147350.016.5781564
GAAGTAC264250.016.51549370
CTACCCT193450.016.4627934
CTAGGAC161950.016.2309443
GTGTAGG177350.016.2226681
Produced by FastQC (version 0.11.5)