FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17434467
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT71800.022.3245244
TACACTG147250.021.5328565
TATACTG71350.021.336545
GTCCTAC71300.021.1349371
CTACACT105550.020.2261284
CCTATAC59200.019.7480623
TATGGCG35950.019.6668516
GTAGGAC73200.019.4618023
TAGACTG86550.018.7620225
CCTACAC103750.018.0157723
CGAATGT16850.017.65550433
TACACAG184600.017.3846665
CGACATC115900.017.21205170
TCTATAC60600.017.0969563
TCTACAC102350.017.0310423
CTAGACT84700.016.8584614
GTGTAGC85550.016.631371
GTATAGC60950.016.5017591
TACACCG18900.016.479995
CTACCCT97850.016.2739054
Produced by FastQC (version 0.11.5)