FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33927763
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAGT523850.022.5277870
ACGAGAG758150.020.78203870
GAGTCGG204700.020.41382270
AGAGTCG264350.018.79952270
CTATACT95150.018.3909744
TACACTG185000.018.3117265
TAGACTG153100.018.2183385
TATACTG91150.018.198995
AGTCGGA119350.017.38881970
CCTATAC87100.017.0794733
CTACACT156050.016.9102944
CGTCTAG33650.016.5590931
GACGAGA994000.016.38618370
CTAGACT150350.016.0616154
GCCGACC88450.015.90618570
GTGTAGG172000.015.5256951
GTATAGG74500.015.5232331
CCTACAC184100.015.4005043
GAGAGTC479100.015.1356670
TACACCG35600.015.0406665
Produced by FastQC (version 0.11.5)