FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27091096
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG12411764.581490538441117RNA PCR Primer, Index 16 (96% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7314372.699916607286763No Hit
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG2114980.7806919291858845RNA PCR Primer, Index 16 (96% over 28bp)
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG1907110.7039619216586882RNA PCR Primer, Index 16 (96% over 28bp)
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG1484540.5479807830587584RNA PCR Primer, Index 16 (96% over 28bp)
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1317090.4861708068215476RNA PCR Primer, Index 16 (96% over 28bp)
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACG683630.2523449032848284RNA PCR Primer, Index 16 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCCGAGCCCACGAGACACCGTGTAG536760.1981315189315338No Hit
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG440720.16268075680659064RNA PCR Primer, Index 16 (96% over 28bp)
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGT363940.13433934160507938RNA PCR Primer, Index 16 (96% over 28bp)
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATTG360980.13324673169369006RNA PCR Primer, Index 16 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA3176800.066.4242334
CTTGAAA3230450.065.95421635
TGCTTGA3161550.065.3765633
TTGAAAA3338000.065.0754636
GCCGTCT3339450.064.9112124
CTGCTTG3130850.064.8197732
CTCGTAT3377500.064.6311517
CGTCTTC3274950.064.5123526
ATGCCGT3495550.064.50979622
TCTCGTA3285400.064.4418716
ATCTCGT3283100.064.3372915
GTCTTCT3224300.064.30324627
TGCCGTC3462600.064.14706423
TATGCCG3562750.063.81091321
CCGTCTT3408950.063.61793525
TCTGCTT3230600.063.56476631
TCTTCTG3264600.063.35788728
TTCTGCT3185750.063.3337530
CGTATGC3631850.063.1713519
GTATGCC3633450.063.0560920
Produced by FastQC (version 0.11.5)