FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr166.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr166.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54472138
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCGATTGACAGGGACAGCCG746620.13706456684332824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGACA173550.031.70242740
GGGACAG184850.029.84017441
CTCGTAT154050.029.19483458
TCTCGTA137750.028.81284157
TCGTATG173300.027.58777859
GACAGGG204200.027.20105737
CAGGGAC204300.027.06782339
TTGACAG220650.025.3793635
ATTGACA227700.024.67046734
ACAGGGA227700.024.51671638
AGCCGAG226950.024.24303446
ATCTCGT163350.023.95450856
GGACAGC257500.021.65225442
TGACAGG263900.021.1669436
GTCTTCT207950.020.68509768
CCCATCT214100.018.2927153
GATTGAC309400.018.24649433
CGTCTAG9350.017.593881
CGTATGC287600.017.45118360
CCGTCTT265450.017.28560466
Produced by FastQC (version 0.11.5)