FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr165.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr165.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49156681
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCGATTGACAGGGACAGCCG1773760.36083803135529025No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGACA286700.047.59819840
GGGACAG294750.046.10824241
CTCGTAT230900.044.8675958
CAGGGAC310750.044.0946239
TCGTATG262000.044.07029759
GACAGGG314700.043.6301337
TCTCGTA199400.043.4425257
TTGACAG330850.041.76489635
ACAGGGA334300.041.14538638
ATTGACA339500.040.82474534
AGCCGAG331700.040.6132246
ATCTCGT219000.038.56365656
GGACAGC363100.037.81438442
TGACAGG369850.037.37029336
GTCTTCT291150.034.6932168
GATTGAC411400.033.6302933
GACAGCC423900.032.5310343
CGTATGC369900.032.44502660
CCCATCT276050.031.37996353
CGATTGA445150.031.08053432