FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64294276
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGATTGACAGGGA4216350.6557893271867623No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCGATTGACAGGGACAGCCG1435350.2232469341438731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT566600.043.46242564
TCTCGTA487850.043.1174363
TCGTATG628750.042.6899365
AGGGACA745900.042.6293846
GGGACAG751050.042.2438747
AGCCGAG770400.040.8693652
CAGGGAC780250.040.8019945
ATCTCGT506650.040.6056362
GACAGGG790250.040.46726243
TTGACAG811300.039.43455541
ACAGGGA809400.039.41901844
ATTGACA824500.038.95603640
GGACAGC833950.038.04874848
TGACAGG860400.037.23297542
CGTATGC766100.036.59872466
CCCATCT580900.036.02399459
GACAGCC893200.035.6462849
GTATGCC788750.035.5078167
CATCTCG586250.035.42659461
GATTGAC914450.035.204539