FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950488
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGACAGGGA289023.040753802257367No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA45180.4753347753995842No Hit
GTCATAAACTCTTCTCTTCCATATGACTATCCCCTTCCCCATTTGGTCTA19390.20400047133682908No Hit
GTCATGGGCTGATTAGACCCGATACCATCGAGATGTCTTATTTAAGGGGA14310.15055424161062528No Hit
GACATACACCATACAGTCATAAACTCTTCTCTTCCATATGACTATCCCCT14180.1491865231333799No Hit
GTCTAGACGCACCTACGGTGAAGAATCATTAGTCCGCAAAACCCAATCAC14140.14876568667884288No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA11960.12583009990657432No Hit
ACATAACTGTGGTGTCATGCATTTGGTATCTTTTTATTTTGGCCTACTTT10790.11352063361136594No Hit
TATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATTG10670.11225812424775483No Hit
GATTAGACCCGATACCATCGAGATGTCTTATTTAAGGGGAACGTATGGGC10370.10910185083872705No Hit
GTATGGTGTATGTCAGATAACACAGATATGTCCTTATAACATTAGTTTAA10190.10720808679331038No Hit
ACACCATACAGTCATAAACTCTTCTCTTCCATATGACTATCCCCTTCCCC9620.10121116731615759No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGG252.8406157E-670.0000165
GTATACG252.8406157E-670.0000150
CGAGAGT252.8406157E-670.0000154
TCGGTAC252.8406157E-670.0000150
GTACGGT252.8406157E-670.0000123
GCGAGAG150.002224785770.028
TCACGAG207.927911E-570.010
CAACGTA301.024091E-770.032
CCGATCG150.002224785770.070
ACGTTAC207.927911E-570.030
TTAGCGG150.002224785770.064
TAGCGAC150.002224785770.034
GACCGAT207.927911E-570.016
ATTCGGC150.002224785770.06
ATATGCG207.927911E-570.013
CGCGAAA207.927911E-570.038
TATGCGA150.002224785770.025
CGTTCGA301.024091E-770.062
AGCGGTA150.002224785770.013
TACGCTA150.002224785770.045
Produced by FastQC (version 0.11.5)