FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr162.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr162.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438153
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGACAGGGA6982915.937126985322477No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA51731.180637813731733No Hit
GACATACACCATACAGTCATAAACTCTTCTCTTCCATATGACTATCCCCT17440.39803447654129953No Hit
GTCATAAACTCTTCTCTTCCATATGACTATCCCCTTCCCCATTTGGTCTA16370.3736137833131349No Hit
GTCTAGACGCACCTACGGTGAAGAATCATTAGTCCGCAAAACCCAATCAC15150.3457696284174706No Hit
GATTTATGTTGATGATTATTTTATGACCTGAACCATTGATTTAATGTACT13280.3030904729626409No Hit
CGGCTATGTTGATGAAAGTAGGCCAAAATAAAAAGATACCAAATGCATGA11120.2537926249506451No Hit
CAGTTATGTTGGTCATGGGCTGATTAGACCCGATACCATCGAGATGTCTT11060.2524232402836452No Hit
CCTTGACGGCTATGTTGATGAAAGTAGGCCAAAATAAAAAGATACCAAAT9950.22708962394414736No Hit
CTTAAACACATTAAACTAATGTTATAAGGACATATCTGTGTTATCTGACA9690.22115562372048123No Hit
ATGACTATCCCCTTCCCCATTTGGTCTATTAATCTACCATCCTCCGTGAA9530.2175039312751482No Hit
CGTATGGGCGATAACGCATTTGATGGCCCTGAAGTAAGAACCAGATGTCT9500.21681923894164823No Hit
GTCATGGGCTGATTAGACCCGATACCATCGAGATGTCTTATTTAAGGGGA9310.21248285416281526No Hit
ACGTATGGGCGATAACGCATTTGATGGCCCTGAAGTAAGAACCAGATGTC8850.20198423838248283No Hit
TATATATACCATGAATATTATCTTAAACACATTAAACTAATGTTATAAGG8710.19878900749281644No Hit
GCATTTATGTCTAACAAGCATGAATAATTAGCCTTAGGTGATTGGGTTTT8350.19057269949081715No Hit
GGATGGTAGATTAATAGACCAAATGGGGAAGGGGATAGTCATATGGAAGA8140.18577985315631754No Hit
GATTGGGTTTTGCGGACTAATGATTCTTCACCGTAGGTGCGTCTAGACTG8100.18486693004498428No Hit
GATTAGACCCGATACCATCGAGATGTCTTATTTAAGGGGAACGTATGGGC7940.18121523759965125No Hit
CCATACAGTCATAAACTCTTCTCTTCCATATGACTATCCCCTTCCCCATT7720.17619416048731837No Hit
ATGCCCCTCTTCTCGCTCCGGGCCCATTAAACTTGGGGGTAGCTAAACTG7270.16592377548481924No Hit
GCCCTGAAGTAAGAACCAGATGTCTGATAAAGTTTCAGTTTAGCTACCCC7180.1638696984843194No Hit
ACATAAATCAATATATATACCATGAATATTATCTTAAACACATTAAACTA6930.15816392903848656No Hit
CTACCATCCTCCGTGAAACCAACAACCCGCCCACCAATGCCCCTCTTCTC6820.1556533904823201No Hit
CCGTAGGTGCGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTAT6730.1535993134818203No Hit
ACCCCCAAGTTTAATGGGCCCGGAGCGAGAAGAGGGGCATTGGTGGGCGG6660.1520016980369871No Hit
CATAAACTCTTCTCTTCCATATGACTATCCCCTTCCCCATTTGGTCTATT6620.1510887749256538No Hit
GACCCGATACCATCGAGATGTCTTATTTAAGGGGAACGTATGGGCGATAA6510.14857823636948736No Hit
TATGGGCGATAACGCATTTGATGGCCCTGAAGTAAGAACCAGATGTCTGA6450.1472088517024875No Hit
GTCCTTTCATGCCTTGACGGCTATGTTGATGAAAGTAGGCCAAAATAAAA6380.1456112362576543No Hit
ATCTGACATACACCATACAGTCATAAACTCTTCTCTTCCATATGACTATC6280.14332892847932116No Hit
GGATAGTCATATGGAAGAGAAGAGTTTATGACTGTATGGTGTATGTCAGA6210.14173131303448797No Hit
CCATATGACTATCCCCTTCCCCATTTGGTCTATTAATCTACCATCCTCCG6210.14173131303448797No Hit
CCTTAAATAAGACATCTCGATGGTATCGGGTCTAATCAGCCCATGACCAA6180.141046620700988No Hit
GCCCATACGTTCCCCTTAAATAAGACATCTCGATGGTATCGGGTCTAATC6160.1405901591453214No Hit
GCCTTAGGTGATTGGGTTTTGCGGACTAATGATTCTTCACCGTAGGTGCG5880.1341996973659886No Hit
GAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGTGCTCT5830.133058543476822No Hit
CTGTGGTGTCATGCATTTGGTATCTTTTTATTTTGGCCTACTTTCATCAA5810.13260208192115538No Hit
GACCAACATAACTGTGGTGTCATGCATTTGGTATCTTTTTATTTTGGCCT5600.1278092355866558No Hit
TCTTCACCGTAGGTGCGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGAC5560.12689631247532254No Hit
CTGTCTCTTATACACATCTCCGAGCCAACGAGACACCGTGTAGACAGGGA5350.12210346614082296No Hit
ACACCATACAGTCATAAACTCTTCTCTTCCATATGACTATCCCCTTCCCC5320.12141877380732302No Hit
ACTTGGGGGTAGCTAAACTGAAACTTTATCAGACATCTGGTTCTTACTTC5100.11639769669499012No Hit
CCCTAGAAGTGATAGTGAGAAGGCTATGGTGAGGTTGAAGAAGGTAGATG5090.11616946591715678No Hit
GCCCATGACCAACATAACTGTGGTGTCATGCATTTGGTATCTTTTTATTT5080.11594123513932347No Hit
TGACTATCCCCTTCCCCATTTGGTCTATTAATCTACCATCCTCCGTGAAA5070.11571300436149015No Hit
GTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAAAGTAGGCCAA4960.1132024658053237No Hit
ATATATACCATGAATATTATCTTAAACACATTAAACTAATGTTATAAGGA4950.11297423502749039No Hit
ATCTTAAACACATTAAACTAATGTTATAAGGACATATCTGTGTTATCTGA4890.1116048503604905No Hit
CATTAAACTTGGGGGTAGCTAAACTGAAACTTTATCAGACATCTGGTTCT4880.1113766195826572No Hit
ATTAATCTACCATCCTCCGTGAAACCAACAACCCGCCCACCAATGCCCCT4870.11114838880482387No Hit
ATATGACTATCCCCTTCCCCATTTGGTCTATTAATCTACCATCCTCCGTG4860.11092015802699057No Hit
ATTTGGTCTATTAATCTACCATCCTCCGTGAAACCAACAACCCGCCCACC4860.11092015802699057No Hit
TCTCGCTCCGGGCCCATTAAACTTGGGGGTAGCTAAACTGAAACTTTATC4810.109779004137824No Hit
CTTGACGGCTATGTTGATGAAAGTAGGCCAAAATAAAAAGATACCAAATG4770.10886608102649074No Hit
GACTATCCCCTTCCCCATTTGGTCTATTAATCTACCATCCTCCGTGAAAC4730.10795315791515749No Hit
GTATAAACATTACTCTGGTCTTGTAAACCTGAAATGAAGATCTTCTCTTC4690.10704023480382424No Hit
GCATTGGTGGGCGGGTTGTTGGTTTCACGGAGGATGGTAGATTAATAGAC4650.10612731169249098No Hit
GTGCGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGAT4610.10521438858115773No Hit
GCTAAACTGAAACTTTATCAGACATCTGGTTCTTACTTCAGGGCCATCAA4600.10498615780332442No Hit
CTTCTCTTCCATATGACTATCCCCTTCCCCATTTGGTCTATTAATCTACC4550.10384500391415784No Hit
CCAATGCCCCTCTTCTCGCTCCGGGCCCATTAAACTTGGGGGTAGCTAAA4540.10361677313632453No Hit
CACCTACGGTGAAGAATCATTAGTCCGCAAAACCCAATCACCTAAGGCTA4510.1029320808028246No Hit
GTCATATGGAAGAGAAGAGTTTATGACTGTATGGTGTATGTCAGATAACA4470.10201915769149134No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATGC1050.070.0000133
TAATCAA401.3460522E-1070.061
GCATAAC207.923152E-570.069
GCGAGAC150.002223983470.066
TGATCCC301.02279955E-770.017
TCACGCG150.002223983470.061
TCGCCGA150.002223983470.010
CTTCGGT301.02279955E-770.032
CGGGTTC252.8380582E-670.025
CTTCGAA301.02279955E-770.016
AACGTGC150.002223983470.029
TAGAGTG353.7071004E-970.026
GTGCAAC500.070.019
AACCGTT252.8380582E-670.017
AACGTAC550.070.040
AAGTAGC301.02279955E-770.065
CTATGCG252.8380582E-670.049
TTAGGCG252.8380582E-670.045
AGCGTAG353.7071004E-970.068
AAGTACA207.923152E-570.027
Produced by FastQC (version 0.11.5)