FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr161.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr161.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160935
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA27650.2381700956556569No Hit
AATTTACATAGTACAACAGTACATTTATGTATATCGTACATTAAACTATT12760.10991140761541345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT1550.045.164825
CGGTGGG6150.039.269671
GTAGTCG400.002419537634.9997241
CGTCTAG5800.033.794291
TGATCGG751.4919478E-632.66640510
CGGATAC552.992103E-431.8206673
TCGGTGT901.7588718E-731.1108630
ACGCATC1052.026718E-829.9997625
CGTAAGG703.491065E-529.9997655
ACACGAC604.9845566E-429.16643324
ACATACG1103.185778E-828.63613570
GCGAAAT3150.027.77755549
TTGACGG7750.027.0965631
CGACGTT657.959755E-426.92286326
GAACGTT657.959755E-426.92286317
CATCGGT1056.777227E-726.66645428
ATCGGTG1056.777227E-726.66645429
TAGACTG11550.026.0626435
CGAAATA3100.025.96753550
TCGGAGA3250.025.8481734
Produced by FastQC (version 0.11.5)