FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25352897
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA277080.1092892855597528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT24650.030.24311664
TCGTATG26450.029.7728765
CGTCTAG76350.026.313091
TCTCGTA25300.024.76260863
ACGGCTA86100.022.72353734
ATGCCGT35950.022.29463869
TTGACGG86450.022.22668331
TATGCCG35050.022.0682668
CGTATGC37450.021.49512166
CGGCTAT90550.021.37476735
CACGAGA536050.021.25900770
TAGACTG159550.020.3139465
GACGGCT98800.019.80259733
TGACGGC98250.019.62846432
CCACGAG770250.019.43895570
CTAGACT153850.019.1781924
CTATACT80300.018.0888164
TAGACGC48950.017.9472774
CTAGACG43000.017.9071923
TCTAGAC170000.017.4590343
Produced by FastQC (version 0.11.5)