FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr159.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr159.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25741895
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA730150.2836426766560892No Hit
GTCTAGACGCACCTACGGTGAAGAATCATTAGTCCGCAAAACCCAATCAC379320.14735511896074474No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG140000.040.525241
TAGACTG226600.030.5681515
GACGGCT185500.030.49039333
ACGGCTA188250.030.1937234
TTGACGG193950.029.25221831
CTAGACT220950.029.1793464
CGGCTAT202000.028.27701435
TGACGGC204850.027.71280332
GACTGTG247000.027.2074347
CTAGACG97350.027.1449073
CTTGACG215150.026.23968330
TAGACGC121500.025.091224
ACTGTGT277050.025.0903638
CCTACGG108000.024.63011412
GACGCAC108900.024.4914576
GTCCGCA109550.024.05737332
CTACGGT110900.024.0490713
ACCTACG112400.023.66612811
AGACTGT306200.023.3762916
AAGATAC224500.023.16686267
Produced by FastQC (version 0.11.5)