FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr156.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr156.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591049
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC9770.16529932374473183No Hit
GGATAATACTATGCCTTCCAGGCATAGTAATGTGGATATTAGGTGAGAGC7830.13247632598989253No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA6780.11471130143186098No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6410.1084512451590308No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA6380.10794367302880133No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG150.00222369670.0053313
TAACACG353.7089194E-969.99940526
AACGAGT252.839428E-669.99940520
TTCGACG150.002224442569.9994054
CGGTGGG5050.061.6826441
GCCGTGT401.0661097E-861.24948552
CCCCTAG308.371295E-658.3377812
TGCGACG308.371295E-658.3377812
TAAGCGA308.375495E-658.3328435
CCAATCG252.3901837E-455.99952315
CGCTAAA750.055.99952329
TCCCGCA252.3901837E-455.99952319
CGATCTA252.3901837E-455.99952362
GTGATCG452.7008355E-854.4439951
GTATTCG407.474191E-752.50413
CGGGTTA407.4785567E-752.49955760
CGTGCCT407.4785567E-752.49955743
TCGATCG200.00695060652.49955745
CACTCGG200.00695060652.49955721
TCGCTAC200.00695060652.49955754
Produced by FastQC (version 0.11.5)