FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr155.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr155.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13283164
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20750.029.3497921
ACGAGAC232950.024.53508670
CACGAGA357700.023.5222970
GTATACG7300.021.0961741
GCTCGAA17150.020.81676311
CCACGAG496650.019.61927270
TACCGAG9300.019.5705997
CGTCTAA16650.019.5498121
TAGACTG69250.019.1559965
GCTACGA16650.018.91874569
TAGACGA18400.018.6419779
CGAGACA147400.018.47337370
CTAGACT68450.018.4593944
ACGGCTA33850.018.40469634
CGTATTA19300.018.31603228
TAGACGC32050.018.2377874
GTATAGG41900.017.959671
GCGTTTA29050.017.95164335
TAACCGT20550.017.88318333
TTGACGG31650.017.8040831
Produced by FastQC (version 0.11.5)