FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr154.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr154.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2701090
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG3000.026.8336871
GCTCGAA3000.026.8331911
CGTACGT3150.026.66652934
CCGTACG2900.026.55158433
CGTACGG2750.024.1816938
CGGTGGG3900.023.3336411
ACGTACG3000.022.1665537
CGTACAC3050.021.8035683
TGCTCGA4000.021.00066810
TTGGACG3750.020.53322417
GACGGCT4300.020.34872833
TAGGGCG3400.019.5587231
CGTCCAA3250.019.38451254
CACGTAC3500.018.999936
GTACGTG4250.018.94107635
TTGACGG4300.018.7208331
TACACCG4550.018.4624675
ACGTCGG950.007213281518.42095454
ACACCGC4000.018.3759236
ACACGTA3650.018.21908235
Produced by FastQC (version 0.11.5)