FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr153.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr153.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9499732
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG11600.022.3275151
TAGACTG44850.020.7585645
ACGGCTA12350.018.98779534
CTAGACT45000.018.8226594
TACACTG52850.018.0136625
TTGACGG12700.017.91332831
CACGAGA55450.017.86288370
CCTATAC23850.017.61013
TATACTG29200.017.5004065
CTATACT33000.017.3943424
TCTAGAC42800.017.3364853
CGGCTAT14500.017.13787835
GTAGGAC33300.016.7117463
CCCACGA101400.016.46444570
ACGAGAC39550.016.01764970
TAGGACG15850.015.8994224
CTACACT43800.015.7423734
GTATACG4450.015.7302871
TACACCG11300.015.4870835
AAGGCGT26150.015.3923266
Produced by FastQC (version 0.11.5)