FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24747943
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG28350.024.197711
ACACCGT127500.020.78025470
TAGACTG118350.019.2823245
TTGACGG30100.019.18603331
TACACTG139700.019.0413285
TATACTG74300.019.031485
ACGGCTA30800.018.86362634
CTATACT76100.018.213274
GACACCG194000.018.14936370
CTACACT116550.018.1084984
CGGCTAT33000.017.71200235
CTAGACT119700.017.690444
TCTAGAC111600.017.4062563
GTATAGG67900.017.0619831
CGAGACA465550.015.9455569
TACACCG24000.015.6045985
TAGGACA109150.015.4881944
GTATAAG58450.015.2695741
GAGACAC491200.015.24114470
CCTACAC136400.015.2165743
Produced by FastQC (version 0.11.5)