FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112529
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC23150.20808446341623454No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG16300.1465130347163984No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTT600.070.0085456
AGCGTTA455.456968E-1270.008546
GACGTAT353.7107384E-970.008546
ATATCGT252.8388768E-670.008546
GTGCCGT455.456968E-1270.008546
CTATGCG550.070.0053949
GATACGA600.070.00539411
AGATACG600.070.00539410
GACGTCG353.7107384E-970.0053945
GGCCGAT455.456968E-1270.00539411
CGACCGA600.070.00539411
TATATCG252.8396335E-670.0053945
ATCGTGC252.8396335E-670.0053948
ATTCCGC700.070.00539410
ACGGATC650.070.0053948
TCGCTCG301.02370905E-770.0053945
GACGGAT650.070.0053947
TCGTATT650.070.0053947
CGTACCA650.070.0053949
CATCGTA150.002224205370.00539411
Produced by FastQC (version 0.11.5)