FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8826239
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG1350.038.88869529
CGCGTAA654.875237E-737.69211632
TCGTATG7300.031.16422365
AATCACG12000.029.16651534
TATGGCG16300.028.34357516
CTATACT37250.028.1885764
CTCGTAT7150.027.41244764
CGTTCGA657.9638086E-426.92294161
ATGGCGA19300.026.83939417
TACACTG74750.026.642915
TTCGTCA8950.026.59204144
TATCGCG1207.4220225E-826.24986664
ATCACGG14200.026.12662735
TTACGCG959.4070965E-625.7894918
CGTTGCG2450.025.71415524
TCGTCAA8450.025.68034260
TATACTG44250.025.6278615
GATTTCG9750.025.48704741
CGTATGC8950.025.02780366
CGACGTA700.001227240924.99987237
Produced by FastQC (version 0.11.5)