FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4218866
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC18300.026.9679643
GTTACGC1251.1013799E-725.2006993
TACACTG37200.023.9928865
TATACTG20900.023.4459045
AAGGCGT9700.023.0936996
TGCGCCA10650.022.67668712
TATGGCG7750.022.58073616
AAAGGCG11250.022.4008875
GTATAGC15650.021.9175421
GTCCTAC19050.021.8641241
AGGCGTG10750.021.8140437
CTACCCT26700.021.6298144
GATTTCG4700.021.59557741
CTACACT26150.021.5493564
CGAATAC1807.2814146E-921.3894823
AATCACG5800.021.12052534
TCTACAC20550.021.1198063
GTGTAGC23950.020.8982851
TAGACCG3700.020.8116385
CGTGCGC7750.020.77476910
Produced by FastQC (version 0.11.5)