FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4922527
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC25000.024.7805583
TATGGCG9700.024.53613516
TATACTG22500.024.1118985
GTCCTAC21050.022.7793771
ATCACGG7450.022.55015635
CTACACT28750.022.157024
GATTTCG6200.022.01595541
AAGGCGT8650.021.8504266
TACACTG39350.021.525485
CTATACT21750.021.4027794
ATGGCGA11950.021.380817
GTGTAGG28550.021.208671
AATCACG7800.021.0897934
TAGGACC22550.020.9539074
TCGTCAA5700.020.87702660
ATTTCGT6750.020.74057642
TAGACTG24400.020.0826235
TACACCG4550.020.0006525
GTATAGG19000.019.8949811
TTCGTCA7050.019.36154744
Produced by FastQC (version 0.11.5)