FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19789661
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG81750.024.3188045
CTATACT77900.023.9928054
TACACTG154400.022.3063965
GTAGGAC88700.022.0182823
CTACACT116150.021.5458724
TATACCG10450.021.4360775
GTCCTAC84750.020.8970381
CCTATAC68850.020.3342843
TCGTCAA22850.019.60594760
TATGGCG36100.019.4877516
GATTTCG25550.019.45187641
TAGGACT89350.018.9987224
TAGACTG101650.018.9725575
TTCGTCA27250.018.62368244
CCTACAC118400.018.5643943
AAGGCGT36650.018.3363196
CTACCCT123000.018.3255924
ATTTCGT27900.018.31524542
GTATAGC71200.017.647711
TCTATAC72800.017.5001933
Produced by FastQC (version 0.11.5)