FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30643006
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG109800.023.0471465
CTATACT104650.022.910284
TACACTG212300.021.1358265
TATACCG14950.020.368515
CTACACT168400.020.181634
TAGACTG154850.019.4387535
GTATAGG98150.018.9355071
ACTTCCG168050.018.93172870
CCTATAC100750.018.6554073
GTAGGAC117950.017.9825043
TAGGACT138100.017.538464
CCTACAC187150.017.4488723
GTCCTAC111300.017.10711
AAGGCGT62800.017.0547686
GTATTAG64500.016.8218841
CTTCCGA146350.016.62099370
GTCCTAG156550.016.566771
TCTACAC160000.016.5377853
TGTACTG191350.016.5173535
GTGTAGG181600.016.4786991
Produced by FastQC (version 0.11.5)