FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17434467
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC70600.021.5158421
TACACTG143600.021.2296735
TATACTG71200.020.843255
CTATACT68300.019.9857754
CTACACT100550.019.9109084
GTAGGAC74700.018.8823723
CGTCTAG15250.018.5903911
TATACCG9500.017.684685
TAGACTG86850.017.651595
TAGGCCG17700.017.5993375
TATGGCG36600.017.11749816
TATACAG107400.017.1093925
TACACCG17250.017.043935
GTGTAGG100650.016.9699041
GTATAAG55800.016.9356921
CCTATAC59800.016.9148753
TCTACAC92400.016.8183383
GTGTAGC95900.016.7885271
GTATAGG57350.016.7831171
TCTATAC58450.016.7666233
Produced by FastQC (version 0.11.5)