FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n01_dpr144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n01_dpr144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33927763
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG88650.020.2149415
TTCCGAT109050.019.73855470
CTTCCGA219500.019.56482370
AGACTTC631450.018.95627870
CTATACT89100.018.8949644
GAGACTT860850.018.7999370
TACACTG186050.018.1919025
CTACACT154350.017.8235664
TAGACTG145750.017.5545675
ACTTCCG289700.016.88980370
CCTATAC83150.016.2480473
TAGACAG160000.016.2098675
CGAGACT1035400.015.74890770
TACACCG34150.015.5788065
CTAGACT144200.015.4858184
GTATAGG82750.015.4382641
TAGGACA139050.015.3042234
TATACAG132100.015.1556475
GACTTCC499350.015.02746170
CCTACAC183300.015.0084633
Produced by FastQC (version 0.11.5)