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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2020-09-16, 05:09 based on data in: /beegfs/mk5636/logs/html/HW5T7BGXF/merged

        General Statistics

        Showing 16/16 rows and 4/5 columns.
        Sample Name% Dups% GCLengthM Seqs
        HW5T7BGXF_n01_ADT1_0909
        2.2%
        48%
        26
        17.8
        HW5T7BGXF_n01_GDO2_0909
        51.7%
        49%
        26
        63.5
        HW5T7BGXF_n01_HTO3_0909
        45.4%
        48%
        26
        21.0
        HW5T7BGXF_n01_cDNA1_0909
        7.6%
        49%
        26
        73.3
        HW5T7BGXF_n01_cDNA2_0909
        10.8%
        49%
        26
        130.2
        HW5T7BGXF_n01_cDNA3_0909
        11.1%
        49%
        26
        132.3
        HW5T7BGXF_n01_cDNA4_0909
        7.7%
        49%
        26
        80.2
        HW5T7BGXF_n01_undetermined
        65.0%
        45%
        26
        22.2
        HW5T7BGXF_n02_ADT1_0909
        2.1%
        43%
        57
        17.8
        HW5T7BGXF_n02_GDO2_0909
        66.1%
        46%
        57
        63.5
        HW5T7BGXF_n02_HTO3_0909
        41.6%
        43%
        57
        21.0
        HW5T7BGXF_n02_cDNA1_0909
        71.2%
        50%
        57
        73.3
        HW5T7BGXF_n02_cDNA2_0909
        73.0%
        50%
        57
        130.2
        HW5T7BGXF_n02_cDNA3_0909
        72.9%
        50%
        57
        132.3
        HW5T7BGXF_n02_cDNA4_0909
        71.4%
        50%
        57
        80.2
        HW5T7BGXF_n02_undetermined
        72.5%
        47%
        57
        22.2

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 8/8 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        22,174,049
        4.1
        cDNA1_0909
        73,340,746
        13.6
        cDNA2_0909
        130,193,933
        24.1
        cDNA3_0909
        132,286,697
        24.5
        cDNA4_0909
        80,241,573
        14.8
        ADT1_0909
        17,751,610
        3.3
        HTO3_0909
        21,029,032
        3.9
        GDO2_0909
        63,461,281
        11.7

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        GGGGGGGG
        16442806.0
        74.2
        GAAAAGAT
        147292.0
        0.7
        CTCTCTAA
        125772.0
        0.6
        CAACACAA
        111113.0
        0.5
        GAAAGGTA
        99826.0
        0.5
        CTCCTTAA
        92841.0
        0.4
        ATCAGATA
        88079.0
        0.4
        CTCCCTAA
        83627.0
        0.4
        TCCTCTAA
        81268.0
        0.4
        GAAGGGTA
        80777.0
        0.4
        CTCCACAA
        73576.0
        0.3
        TGTAAACA
        67325.0
        0.3
        GGGGGGGT
        61872.0
        0.3
        TGGTAACA
        57704.0
        0.3
        TATAAAAA
        57039.0
        0.3
        CCCTCTAA
        54379.0
        0.2
        GATCAATA
        53590.0
        0.2
        GAAACGCT
        53104.0
        0.2
        AATCATAT
        51726.0
        0.2
        GCCTTGTG
        51652.0
        0.2

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined% PhiX Aligned
        4.0
        606,355,344
        540,478,921
        4.1
        2.6

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (57bp , 26bp). See the General Statistics Table.

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

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        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        No samples found with any adapter contamination > 0.1%

        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.