FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22174049
Sequences flagged as poor quality0
Sequence length57
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11067304.991104691795351No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1373680.6194989467192031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCTC875100.050.541163
CATAGCT878300.050.4440352
TAGCTCT873550.050.3223084
GCATAGC884600.050.176261
ACGGAAC122350.048.39961
TTAAACG86150.048.17569710
GTTACCG64100.047.843595
CTAGTCC65150.047.7385711
CGGAACA124300.047.638272
CCTAGTC66200.047.25148410
ACCTAGT65800.046.7646989
TAGATCG76650.046.59253751
TTAGAGT73450.046.4720883
TAAACCG113100.046.3430411
ACCCGTC57350.046.0492861
GGACCAT63850.046.030572
CCGCGTA38950.045.68028615
GAGATCG111600.045.6243651
CGTCCCA66650.045.5937210
CACCTAG67700.045.4526528
Produced by FastQC (version 0.11.5)