FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n02_cDNA4_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n02_cDNA4_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80241573
Sequences flagged as poor quality0
Sequence length57
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9441941.176689295460347No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2169410.27035985448590344No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330640.16582925162745749No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1208250.15057655960956798No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1193590.1487495764820064No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG853260.1063363999606538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2570350.046.9692341
AGCAGTG2846550.042.3580632
CAGTGGT3541050.034.0005874
ATCAACG3625700.033.1649511
TGGTATC3633700.033.0474627
GTATCAA3657900.032.8525359
TCAACGC3659650.032.8290812
AGTGGTA3671450.032.816725
CAACGCA3668500.032.7830713
TATCAAC3674550.032.77282310
AACGCAG3694250.032.5861414
GGTATCA3691300.032.5401768
GCAGTGG3713200.032.5342643
ACGCAGA3700900.032.5196815
AGAGTAC3704650.032.3886319
GTGGTAT3756300.032.0199746
CAGAGTA3769650.031.94133618
GCAGAGT3762400.031.93289417
GAGTACT3741450.031.8883720
CGCAGAG3785550.031.79590416