Basic Statistics
Measure | Value |
---|---|
Filename | HW5T7BGXF_n02_cDNA4_0909.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 80241573 |
Sequences flagged as poor quality | 0 |
Sequence length | 57 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 944194 | 1.176689295460347 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 216941 | 0.27035985448590344 | No Hit |
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 133064 | 0.16582925162745749 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA | 120825 | 0.15057655960956798 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA | 119359 | 0.1487495764820064 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG | 85326 | 0.1063363999606538 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAGCAGT | 257035 | 0.0 | 46.969234 | 1 |
AGCAGTG | 284655 | 0.0 | 42.358063 | 2 |
CAGTGGT | 354105 | 0.0 | 34.000587 | 4 |
ATCAACG | 362570 | 0.0 | 33.16495 | 11 |
TGGTATC | 363370 | 0.0 | 33.047462 | 7 |
GTATCAA | 365790 | 0.0 | 32.852535 | 9 |
TCAACGC | 365965 | 0.0 | 32.82908 | 12 |
AGTGGTA | 367145 | 0.0 | 32.81672 | 5 |
CAACGCA | 366850 | 0.0 | 32.78307 | 13 |
TATCAAC | 367455 | 0.0 | 32.772823 | 10 |
AACGCAG | 369425 | 0.0 | 32.58614 | 14 |
GGTATCA | 369130 | 0.0 | 32.540176 | 8 |
GCAGTGG | 371320 | 0.0 | 32.534264 | 3 |
ACGCAGA | 370090 | 0.0 | 32.51968 | 15 |
AGAGTAC | 370465 | 0.0 | 32.38863 | 19 |
GTGGTAT | 375630 | 0.0 | 32.019974 | 6 |
CAGAGTA | 376965 | 0.0 | 31.941336 | 18 |
GCAGAGT | 376240 | 0.0 | 31.932894 | 17 |
GAGTACT | 374145 | 0.0 | 31.88837 | 20 |
CGCAGAG | 378555 | 0.0 | 31.795904 | 16 |