Basic Statistics
Measure | Value |
---|---|
Filename | HW5T7BGXF_n02_cDNA3_0909.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 132286697 |
Sequences flagged as poor quality | 0 |
Sequence length | 57 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1486375 | 1.1236012643055107 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 318484 | 0.2407528551415869 | No Hit |
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 211089 | 0.1595693329617263 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA | 190662 | 0.14412787099824556 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA | 189530 | 0.1432721538130172 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG | 136903 | 0.1034896199728987 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAGCAGT | 413630 | 0.0 | 46.870544 | 1 |
AGCAGTG | 457975 | 0.0 | 42.28258 | 2 |
CAGTGGT | 568150 | 0.0 | 33.987904 | 4 |
ATCAACG | 581510 | 0.0 | 33.212307 | 11 |
TGGTATC | 582925 | 0.0 | 33.04749 | 7 |
GTATCAA | 586935 | 0.0 | 32.880795 | 9 |
TCAACGC | 587455 | 0.0 | 32.864796 | 12 |
TATCAAC | 589675 | 0.0 | 32.811104 | 10 |
AGTGGTA | 589460 | 0.0 | 32.80489 | 5 |
CAACGCA | 589615 | 0.0 | 32.78338 | 13 |
AACGCAG | 593670 | 0.0 | 32.596592 | 14 |
GGTATCA | 591895 | 0.0 | 32.576523 | 8 |
ACGCAGA | 594470 | 0.0 | 32.525833 | 15 |
GCAGTGG | 597105 | 0.0 | 32.470356 | 3 |
AGAGTAC | 594585 | 0.0 | 32.393463 | 19 |
GTGGTAT | 603595 | 0.0 | 31.967617 | 6 |
CAGAGTA | 605825 | 0.0 | 31.904345 | 18 |
GAGTACT | 600375 | 0.0 | 31.89466 | 20 |
GCAGAGT | 605040 | 0.0 | 31.887285 | 17 |
GTACTTT | 597840 | 0.0 | 31.84519 | 22 |