FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n02_cDNA3_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n02_cDNA3_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132286697
Sequences flagged as poor quality0
Sequence length57
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14863751.1236012643055107No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3184840.2407528551415869No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110890.1595693329617263No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1906620.14412787099824556No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1895300.1432721538130172No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1369030.1034896199728987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4136300.046.8705441
AGCAGTG4579750.042.282582
CAGTGGT5681500.033.9879044
ATCAACG5815100.033.21230711
TGGTATC5829250.033.047497
GTATCAA5869350.032.8807959
TCAACGC5874550.032.86479612
TATCAAC5896750.032.81110410
AGTGGTA5894600.032.804895
CAACGCA5896150.032.7833813
AACGCAG5936700.032.59659214
GGTATCA5918950.032.5765238
ACGCAGA5944700.032.52583315
GCAGTGG5971050.032.4703563
AGAGTAC5945850.032.39346319
GTGGTAT6035950.031.9676176
CAGAGTA6058250.031.90434518
GAGTACT6003750.031.8946620
GCAGAGT6050400.031.88728517
GTACTTT5978400.031.8451922