FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n02_cDNA2_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n02_cDNA2_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130193933
Sequences flagged as poor quality0
Sequence length57
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15135431.1625295934488744No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3519980.27036436482796783No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130710.16365662753271307No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1924470.14781564360606572No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1907810.146536014086002No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1348500.1035762549703449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4155800.046.9341771
AGCAGTG4590500.042.4287722
CAGTGGT5693450.034.148154
ATCAACG5816750.033.40199711
TGGTATC5831900.033.235277
GTATCAA5868550.033.0914279
AGTGGTA5891150.033.0262575
TCAACGC5882650.033.0165112
TATCAAC5901550.032.9759110
CAACGCA5905900.032.92705513
AACGCAG5936700.032.78167714
GGTATCA5919750.032.7719088
GCAGTGG5955900.032.7660333
ACGCAGA5945750.032.71943315
AGAGTAC5947250.032.587719
GTGGTAT6027250.032.1994556
GCAGAGT6045400.032.1178817
CAGAGTA6058500.032.09835418
GAGTACT6010500.032.06455220
GTACTTT5985250.032.03141422