Basic Statistics
Measure | Value |
---|---|
Filename | HW5T7BGXF_n02_cDNA2_0909.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 130193933 |
Sequences flagged as poor quality | 0 |
Sequence length | 57 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1513543 | 1.1625295934488744 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 351998 | 0.27036436482796783 | No Hit |
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 213071 | 0.16365662753271307 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA | 192447 | 0.14781564360606572 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA | 190781 | 0.146536014086002 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG | 134850 | 0.1035762549703449 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAGCAGT | 415580 | 0.0 | 46.934177 | 1 |
AGCAGTG | 459050 | 0.0 | 42.428772 | 2 |
CAGTGGT | 569345 | 0.0 | 34.14815 | 4 |
ATCAACG | 581675 | 0.0 | 33.401997 | 11 |
TGGTATC | 583190 | 0.0 | 33.23527 | 7 |
GTATCAA | 586855 | 0.0 | 33.091427 | 9 |
AGTGGTA | 589115 | 0.0 | 33.026257 | 5 |
TCAACGC | 588265 | 0.0 | 33.01651 | 12 |
TATCAAC | 590155 | 0.0 | 32.97591 | 10 |
CAACGCA | 590590 | 0.0 | 32.927055 | 13 |
AACGCAG | 593670 | 0.0 | 32.781677 | 14 |
GGTATCA | 591975 | 0.0 | 32.771908 | 8 |
GCAGTGG | 595590 | 0.0 | 32.766033 | 3 |
ACGCAGA | 594575 | 0.0 | 32.719433 | 15 |
AGAGTAC | 594725 | 0.0 | 32.5877 | 19 |
GTGGTAT | 602725 | 0.0 | 32.199455 | 6 |
GCAGAGT | 604540 | 0.0 | 32.11788 | 17 |
CAGAGTA | 605850 | 0.0 | 32.098354 | 18 |
GAGTACT | 601050 | 0.0 | 32.064552 | 20 |
GTACTTT | 598525 | 0.0 | 32.031414 | 22 |