Basic Statistics
Measure | Value |
---|---|
Filename | HW5T7BGXF_n02_cDNA1_0909.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 73340746 |
Sequences flagged as poor quality | 0 |
Sequence length | 57 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 826945 | 1.1275382991059295 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 202388 | 0.2759557422554714 | No Hit |
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 117882 | 0.16073193474197822 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA | 105458 | 0.14379182889685904 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA | 104470 | 0.14244469234059878 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG | 77798 | 0.10607745931572607 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAGCAGT | 228845 | 0.0 | 46.89592 | 1 |
AGCAGTG | 253785 | 0.0 | 42.21603 | 2 |
CAGTGGT | 315875 | 0.0 | 33.87673 | 4 |
ATCAACG | 323710 | 0.0 | 33.046658 | 11 |
TGGTATC | 324290 | 0.0 | 32.907238 | 7 |
GTATCAA | 326645 | 0.0 | 32.722996 | 9 |
TCAACGC | 326895 | 0.0 | 32.72211 | 12 |
AGTGGTA | 327230 | 0.0 | 32.71888 | 5 |
CAACGCA | 327850 | 0.0 | 32.652443 | 13 |
TATCAAC | 327865 | 0.0 | 32.65002 | 10 |
AACGCAG | 329965 | 0.0 | 32.47861 | 14 |
ACGCAGA | 330325 | 0.0 | 32.4262 | 15 |
GGTATCA | 329335 | 0.0 | 32.42334 | 8 |
AGAGTAC | 330610 | 0.0 | 32.261326 | 19 |
GCAGTGG | 333100 | 0.0 | 32.25041 | 3 |
GTGGTAT | 334465 | 0.0 | 31.938868 | 6 |
CAGAGTA | 336215 | 0.0 | 31.846674 | 18 |
GCAGAGT | 336225 | 0.0 | 31.809412 | 17 |
GAGTACT | 333975 | 0.0 | 31.746653 | 20 |
GTACTTT | 332470 | 0.0 | 31.72485 | 22 |