FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n02_cDNA1_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n02_cDNA1_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73340746
Sequences flagged as poor quality0
Sequence length57
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8269451.1275382991059295No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2023880.2759557422554714No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1178820.16073193474197822No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1054580.14379182889685904No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1044700.14244469234059878No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG777980.10607745931572607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2288450.046.895921
AGCAGTG2537850.042.216032
CAGTGGT3158750.033.876734
ATCAACG3237100.033.04665811
TGGTATC3242900.032.9072387
GTATCAA3266450.032.7229969
TCAACGC3268950.032.7221112
AGTGGTA3272300.032.718885
CAACGCA3278500.032.65244313
TATCAAC3278650.032.6500210
AACGCAG3299650.032.4786114
ACGCAGA3303250.032.426215
GGTATCA3293350.032.423348
AGAGTAC3306100.032.26132619
GCAGTGG3331000.032.250413
GTGGTAT3344650.031.9388686
CAGAGTA3362150.031.84667418
GCAGAGT3362250.031.80941217
GAGTACT3339750.031.74665320
GTACTTT3324700.031.7248522