FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n02_HTO3_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n02_HTO3_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21029032
Sequences flagged as poor quality0
Sequence length57
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG649990.3090917356538332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAC4235600.050.6834951
CATGTTA3847500.050.6701052
TTACTAT4242900.050.654734
TATCCCC4187950.050.6134878
TACTATC4225650.050.6111455
TCGATAA2031450.050.603841
CAAGAAC6687250.050.593687
CTTACTA4245350.050.591013
AGGACCA3721900.050.589531
GCTTACT4237800.050.5855672
ACATGTT3857600.050.5850371
TGTTACC3815000.050.582724
CCGTCCC3757300.050.5743649
ATCCCCC4164350.050.5572059
CATCCAA3706700.050.5561646
CTATCCC4198150.050.556137
ATGTTAC3854700.050.5492363
CCATCCA3710350.050.533575
GGACCAT3718200.050.5239562
ATCCAAC3722150.050.5202377
Produced by FastQC (version 0.11.5)