FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n02_GDO2_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n02_GDO2_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63461281
Sequences flagged as poor quality0
Sequence length57
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3918640.6174851717852969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGCT54693000.050.9740372
ATAGCTC54704600.050.9495773
TAGCTCT54688900.050.9437184
AGCTCTT54625750.050.9232865
GCTCTTA54629200.050.923016
TCTTAAA54688800.050.9048358
CTCTTAA54671250.050.897997
CTTAAAC54655700.050.8902329
TTAAACT21681550.050.8394910
TAAACTT12739300.050.8003511
TTAAACA13910350.050.7992910
TTAAACC14068900.050.7891810
CGTCCCT1862700.050.710054
TTAAACG5214500.050.70633710
CTTTTGC2400600.050.6954415
TAAACTC5355450.050.68546311
CCCGTCC1867350.050.646032
ACTTTCT3461050.050.64275714
TAAACCG6300850.050.64218511
TTTCTAA1741800.050.6318916
Produced by FastQC (version 0.11.5)