FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n02_ADT1_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n02_ADT1_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17751610
Sequences flagged as poor quality0
Sequence length57
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG581020.32730552327366363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC3033350.050.8153882
ACCCGTC3038150.050.8141561
CCGTCCC3027450.050.8037153
CGTCCCT3031500.050.792794
CGTAGTC2181600.050.7370382
TAGAGTC4322900.050.7124024
CCTCATT1232350.050.699024
TTAGAGT4314200.050.6976283
CCCTAAG3056750.050.635967
AGAGTCT4325400.050.6344155
TCCCTAA3057950.050.6206676
TTCCTCA1234400.050.606272
GTCCCTA3058200.050.6020665
TGCCACC4251600.050.59046611
TCTGCCA4261500.050.584159
AGTCTGC4298600.050.5633477
CTAGTCC2171450.050.56052811
GCCACCC4235900.050.55542812
CTGCCAC4261300.050.54860710
GAGTCTG4321200.050.5481576
Produced by FastQC (version 0.11.5)