FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22174049
Sequences flagged as poor quality0
Sequence length26
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG441440.19907956368275365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT15650.016.882571
ATGCGAT18050.016.8640771
TGCGGGT15750.016.7753771
ATGGGAG19000.016.7082371
CCGGGAT13100.016.4111731
ACGAGCC15750.016.26511
ACAGCCG23050.016.25681
GACTGCG19050.016.1897641
GGGAGAT14800.016.0194441
CGGAGCT19550.015.9298641
CTGAAGT21550.015.8499821
GACCTGG16000.015.7598071
GGCTCGA20400.015.709331
TGGGCGT12350.015.699561
AGCTGAA12350.015.6223868
ATCGAGT20200.015.6162031
GGCCCTA10950.015.61126210
GCGCGAT11400.015.5978791
CGGACGT15950.015.557271
CCTCTGA27500.015.4892481
Produced by FastQC (version 0.11.5)