FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n01_cDNA4_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n01_cDNA4_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80241573
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA295350.017.615518
ACTGAAC244550.017.461051
ATGCGAT206350.017.0530951
GGGACCT199350.017.049791
GAATGAA209650.016.7560461
TGACGGC192700.016.7401711
ATCGAGT240150.016.727191
AGCTTGA275500.016.7121091
CCGGGAT154000.016.6666321
GACTAAC207250.016.6556761
ACTGATG183300.016.6491321
TGCGGGT201200.016.6295321
GACCTGG221900.016.6153661
CTGAAGT230850.016.5258061
ATGGGAG230200.016.4986021
CTCGAAA176400.016.4951781
CGGACTG179700.016.4705751
GCGCGAT126700.016.452541
GGAAAGC195800.016.3014411
ACGAGCC221200.016.2971461
Produced by FastQC (version 0.11.5)