FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n01_cDNA3_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n01_cDNA3_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132286697
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA487100.017.4493798
ACTGAAC403550.017.3678911
GGGACCT330300.017.1343141
ATCGAGT391300.017.0501561
ATGCGAT350550.017.0262261
TGACGGC328200.016.8812711
GAATGAA347050.016.7166121
CGGACTG305050.016.6540371
CCGGGAT262050.016.6538031
GACCTGG365650.016.6376951
AGCTTGA449800.016.6272431
GGAAAGC319100.016.5758931
TGCGGGT316800.016.5446821
ATGGGAG384150.016.529071
CTGAAGT385350.016.5191291
GCGCGAT219800.016.414621
ACTGATG298450.016.40231
GACTAAC345850.016.3668171
CGCTGGA288950.016.3461551
GATGCTA342350.016.2857951
Produced by FastQC (version 0.11.5)