FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n01_cDNA2_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n01_cDNA2_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130193933
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA469900.017.5879178
ACTGAAC387500.017.3127961
GGGACCT328850.016.996881
ATGCGAT352200.016.8981131
ATCGAGT393400.016.8421131
GAATGAA345250.016.7834191
ATGGGAG385200.016.7617991
GACCTGG359350.016.7567391
TGACGGC327700.016.7543431
AGCTTGA454950.016.6566221
CCGGGAT253150.016.654451
CGGACTG298200.016.637371
GACTAAC339050.016.5771
TGCGGGT318050.016.4481451
GGAAAGC307000.016.4243771
ACTGATG299250.016.3918111
CTGAAGT371900.016.3445781
TGCCAAA388750.016.2793881
GAAATGA349850.016.2539621
GCGCGAT219900.016.2387891
Produced by FastQC (version 0.11.5)