FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n01_cDNA1_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n01_cDNA1_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73340746
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA270500.017.4742328
ACTGAAC223700.017.429611
ATGCGAT195050.017.082071
GGGACCT190150.017.0540891
GAATGAA187000.016.9669341
TGACGGC180950.016.9648531
ATCGAGT223600.016.8782271
GACCTGG202800.016.7794691
CGGACTG167350.016.7118021
TGCGGGT178750.016.7091961
AGCTTGA257350.016.658651
CCGGGAT146350.016.6356471
CTGAAGT217700.016.5637511
ATGGGAG211350.016.5171451
ACTGATG165800.016.469861
GGAAAGC172700.016.3504751
GACTAAC190750.016.3397241
TCCCGAT213900.016.3249131
CGCTGGA161400.016.2867221
CATGGCG218850.016.2618981
Produced by FastQC (version 0.11.5)