FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n01_HTO3_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n01_HTO3_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21029032
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAA95250.018.9411855
TCCCGTA97950.018.6742784
GGCCCTA51200.018.45599710
AACTCCC133000.018.208181
ACTCCCG107000.018.1042882
CTCCCGT107150.018.0602743
GTAAATG124800.017.9637378
AAATGTG112900.017.89093810
TAAATGT114250.017.6620739
TTGACGA68150.017.59255810
CATGGCG82850.017.5306281
CAATGGA57150.017.4793368
GGGACCT41750.017.442081
GAATGAA48350.017.3782561
CGTAAAT113100.017.3467167
ATAAGAG132650.017.3136251
TGACGGC51550.017.2502921
CTGAAAC50450.017.1902161
CCCATAC38750.017.1145381
AGGTCCC66200.016.9349216
Produced by FastQC (version 0.11.5)