FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n01_GDO2_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n01_GDO2_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63461281
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACA301150.017.869763
GAAATGA255400.017.7376481
CTGACAC288650.017.6907734
ACTGAAC203900.017.68491
CTAATGG281200.017.6113321
ATGGGAG211500.017.5649171
CTCTGAC306750.017.5630932
CAATGGA187950.017.525018
CGGACTG201550.017.4841461
GGCCCTA239100.017.35579310
TGCGGGT208700.017.282951
TTGTAGG247200.017.2820661
TGACGGC192900.017.2777561
CCTCTGA414850.017.2734491
ATGCGAT153650.017.22551
CCGTGGA246550.017.120721
GTTGATT256100.017.101798
TCTGGAA230050.017.0964471
ATGAATG147250.017.03810710
GTTGGAC167450.017.0131348
Produced by FastQC (version 0.11.5)