FastQCFastQC Report
Wed 16 Sep 2020
HW5T7BGXF_n01_ADT1_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5T7BGXF_n01_ADT1_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17751610
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT77700.018.1127261
CACTGAG67300.017.8171312
GTTGGAC51450.017.6862038
CAATGGA55650.017.6810688
CATGGCG90350.017.6691421
ACACTGA115650.017.626631
TTGTAGG84900.017.5427531
ACTGAAC60500.017.4920621
ATGGCGC63200.017.4538842
ATGAATG55000.017.45373310
TCTGACA71300.017.4487573
TCTGGAA76850.017.4410111
GGGACCT35650.017.3957391
CTGACAC69250.017.3730894
TGGACCC52500.017.35157610
GGCCCTA36300.017.32701710
CCTCTGA97850.017.3268491
CCCATTG22150.017.2924866
ATGGGAG45650.017.178531
GACCAAT87250.017.139041
Produced by FastQC (version 0.11.5)