FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah9847i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah9847i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7649881
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC10250.021.20538344
GTCGCCG12050.018.03765945
CGCCGTA13650.016.17652747
TGGTCGC14450.015.519343
CTCGGTG14500.014.9876638
GTGGTCG15500.013.799787542
ATCTCGG16400.013.46127136
CGTGTAG18700.013.28139516
TCGCCGT17150.012.87477546
AGCGTCG20400.012.51099511
TGTAGGG20800.012.27224418
GATCTCG17800.012.20920935
GTGTAGG21550.012.16511617
GTCGTGT20700.012.16313914
CCGTATC19600.011.97076949
GCGTCGT20850.011.74464212
GTAGGGA21350.011.6329619
CGTCGTG21350.011.63113513
GAGCGTC22100.011.392410
GCCGTAT20550.011.08149948
Produced by FastQC (version 0.11.2)