FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah9847c3hmyc-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah9847c3hmyc-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6675544
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC7850.026.37063444
CGCCGTA10050.019.9116647
CTCGGTG10750.019.89381638
GTGGTCG11100.018.95819742
TGGTCGC11200.018.78977243
GTCGCCG12400.017.5290345
AGCGTCG12900.016.84317211
GCGTCGT13650.016.42292412
ATCTCGG12450.015.79211736
CGTCGTG14750.015.66556413
CCGTATC14100.015.41756549
GTCGTGT14400.015.32863714
CGTGTAG13350.015.24411916
GTGTAGG15150.014.11596817
TCGCCGT14000.014.04707246
GAGCGTC15900.013.88243510
GATCTCG15100.013.70708135
GCCGTAT15950.012.98011148
GGTGGTC16850.012.89796241
TCGGTGG18200.012.88789939
Produced by FastQC (version 0.11.2)