FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah9767i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah9767i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7247956
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC7600.016.34254344
CGCCGTA10900.011.39487847
TGGTCGC12050.010.87905743
GTCGCCG12750.010.5531545
CTCGGTG12100.09.97760838
GGGGGGA20150.09.9375772
ATCTCGG12700.09.77735936
GTGGTCG12800.09.70217842
TCGCCGT15800.08.7343546
GGGGGAG18700.08.1201623
CGTGTAG15500.08.011126516
CCGTATC17300.07.978248649
GATCTCG15700.07.909346635
AGCGTCG18450.07.851158611
GCGTCGT18900.07.66406712
GTCGTGT17100.07.462536314
GTAGGGA19000.07.44313719
TCTCGGT18350.07.33069837
GCCGTAT18400.07.12612548
GGTGGTC19500.07.07608641