FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah9767c3htop2-spikein-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah9767c3htop2-spikein-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6552917
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG67090.1023818858074961Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC20000.046.74790244
CGCCGTA22800.040.25065647
CTCGGTG22800.039.4871338
TGGTCGC23950.039.46856343
GTGGTCG24550.038.78352742
GTCGCCG25050.037.46111745
GTCGTGT26300.037.2442714
GCGTCGT26450.036.9015312
AGCGTCG26500.036.57160611
CGTGTAG26900.035.64805216
ATCTCGG25500.034.89845736
GATCTCG25800.034.89613735
TCGCCGT26600.034.88913346
GGTGGTC27000.034.87990641
CCGTATC27150.034.3138849
TGTAGGG28600.033.89145318
GTGTAGG28400.033.52231617
TCGGTGG27750.033.19108239
GAGCGTC29950.032.81897410
CGTCGTG30450.032.39383313
Produced by FastQC (version 0.11.2)