FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah9624i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah9624i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8626279
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC14350.030.77553244
CGCCGTA18100.024.01814547
GTCGCCG18450.023.37537645
CTCGGTG19450.022.69953538
TGGTCGC19600.022.53051843
GTGGTCG20550.021.82409342
ATCTCGG21600.019.80053336
CGTGTAG24350.019.26377316
GTCGTGT25600.018.99416414
GATCTCG22550.018.50911135
GTAGGGA25500.018.39554819
AGCGTCG25950.018.07340611
TCGCCGT24250.017.92663446
CCGTATC25200.017.66422549
GCGTCGT27000.017.62589812
GCCGTAT25450.017.35461848
TGTAGGG27500.017.30853818
CGTCGTG28000.016.87304513
GTGTAGG28450.016.60904917
TCTCGGT28150.015.68286637
Produced by FastQC (version 0.11.2)