FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah9624c3hflag-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah9624c3hflag-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7155206
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC11150.033.72756244
GTCGCCG12500.030.36099245
CGCCGTA13050.029.0818247
TGGTCGC14200.026.96787343
GTGGTCG14550.026.08059142
CTCGGTG14650.024.0150538
GTCGTGT17900.022.7348614
CGTGTAG17750.022.15196616
ATCTCGG17150.021.31760636
GCGTCGT18650.021.26582512
TCGCCGT17550.021.23131446
AGCGTCG18550.021.1945511
CGTCGTG19600.021.11398713
GATCTCG17650.020.32572435
GCCGTAT20200.019.64475448
CCGTATC19050.019.56325349
TGTAGGG20100.019.04753318
GTGTAGG20200.018.78222817
GTAGGGA20600.018.75251819
GAGCGTC21300.018.61955810
Produced by FastQC (version 0.11.2)